This study was performed to evaluate adverse toxicological effects mediated by transactivation and transrepression mechanism on oral administration of Prednisolone in female ICR Mice. Twenty four female ICR mice of 5-7 weeks old were randomly divided in two groups (Group I and Group II); 12 animals in each group. Animals of Group I served as vehicle control and Group II as Prednisolone treated group at 40 mg/kg body weight per day for 14 consecutive days. Test item was formulated in distilled water and administered orally with gavage needle fitted on the graduated syringe. Animals of control group were administered with vehicle alone (Elix water) and handled in same manner as treated group. Animal were observed for clinical signs of toxicological significance and mortality, body weight, body weight changes, feed consumption, hematology and clinical chemistry investigations, organ weight and relative organ weight parameters. Significant decrease in body weight was observed on day 7 onwards till the end of treatment period in treated group as compared to concurrent control group; however significant reduction in body weight gain was observed in treated group on day 4 and day 7 of the treatment period. Feed consumption was comparatively more in treated group of animals compared to concurrent control group but this change was statistically not significant. Significant decrease in WBC, MCV, lymphocyte and basophils; increase in RBC, Hb, MCHC and neutrophils were observed in treated group compared to concurrent control group of animals. Changes noticed in hematological profile are considered to be adverse effect of drug on bone marrow leading to altered leucopoiesis and erythropoiesis. Significant increase in serum glucose, triglyceride, total cholesterol, HDL cholesterol, total protein, albumin and calcium were observed in treated group compared to concurrent control group of animals. Significant decrease in urea level was observed in treated animals as compared to concurrent control group. Changes noticed in serum biochemical profile are indicative of catabolic effects of glucocorticoid on body system. Significant reduction in spleen, thymus and lungs absolute organ weight was observed in treated mice compared to concurrent control group. Changes in absolute organ weight are indicative of immunosuppressive effect of prednisolone on treated animals. Significant reduction in spleen and thymus relative organ weight was observed in treated mice compared to concurrent control group of animals. Changes observed in relative organ weight are indicative of immunosuppressive effect of prednisolone on treated animals.
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